Precautions for free human S100B protein ELISA test kit:

1. The kit should be balanced at room temperature for 15-30 minutes before use when taken out from the cold storage environment. If the enzyme coated plate is not used up after opening, the Flat noodles should be stored in a sealed bag.

2. There may be crystal precipitation in concentrated washing solution. When diluting, it can be dissolved by heating in a water bath, and washing does not affect the results.

3. Sample dispensers should be used for each step of sample addition, and their accuracy should be regularly checked to avoid experimental errors. It is recommended to control the sample addition time within 5 minutes. If there are a large number of specimens, it is recommended to use a sampling gun for sample addition.

4. The sealing film is only for one-time use to avoid cross contamination.

5. Please store the substrate away from light.

6. Strictly follow the instructions for operation, and the judgment of the test results must be based on the reading of the enzyme-linked immunosorbent assay reader

7. All samples, detergents, and various waste materials should be treated as infectious agents.

8. Components from different batch numbers of this reagent must not be mixed.

If proxy testing is required or the sample is temporarily not tested, it can be immediately frozen at low temperature. The lower the temperature, the better, and repeated freezing and thawing should be avoided in between. It can be stored for three months below -20 ℃ and six months below -70 ℃.

10. Instructions for using reagents:

11. (1) Please refer to relevant regulations, literature, MSDS and other information to understand and master the characteristics of the reagent before carrying out safe operations.

12. (2) Usually, when purchasing reagents, it is important to consider using them all at once. If there is a surplus due to insufficient estimation, pay more attention to other storage and management.

13. (3) In case the reagent operator is not a professional technician. Operations must be carried out under the guidance and supervision of professionals. For waste or old reagents after use, they should be disposed of in accordance with relevant laws and regulations.

14. (4) After purchase, please make sure to confirm the precautions on the label; Take measures and management systems to prevent inversion and breakage; Confirm the precautions on the label again before use and take necessary safety measures; Operations that do not indicate their hazardous or harmful characteristics should also be carried out with caution; It is necessary to wear protective equipment and operate carefully.