【Product Name】 ：Gelatin Enzyme Spectrum Detection Kit

：50T

[Storage]:According to the instructions, please refer toSave at 4 degrees and 20 degrees

:6month

【Product Introduction】 ：

Matrix metalloproteinases Matrix metalloproteinases (MMPs) belong to the family of metalloproteinases, which consists of at least 20 members and are known to be involved in the metabolism of extracellular matrix proteins. They secrete in the form of inactive zymogens, which can degrade extracellular matrix collagen, also known as collagenase, after activation. By affecting the extracellular matrix, collagenase is involved in embryonic development, morphogenesis, tissue remodeling, and many other disease processes such as inflammation, tumors, cardiovascular disease, and neurological disorders. MMP-2 and MMP-9 in plasma and tissues are involved in various physiological and pathological processes, and their significance in clinical diagnosis and treatment is increasingly being recognized. MMP can be widely detected by enzyme-linked immunosorbent assay (ELISA). The MMP2&MMP9 Gelatin Zymography Kit provides a simple enzymatic electrophoresis system for detecting the enzymatic profiles and activities of MMP2 and MMP9 in samples such as blood, body fluids, secretions, cell lysates, and cell culture media.

This product is only for scientific research experiments and is not intended for any other purposes!

【test kitComposition and Storage】：

A. 2 × SDS-PAGE non-reducing buffer 1.5 ml, −20 ºC 保存；

B. 10 × Substrate G, 50 ml, −20 ºC 保存；

C. 10 x Buffer A, 50 ml, stored at 4 º C, diluted with distilled water when in use;

D. 10 x Buffer B, 50 ml, stored at 4 º C, diluted with distilled water when in use;

E. SDS-PAGE gel protein Coomassie brilliant blue staining solution, 50ml, stored at 4 º C.

【Precautions】：

10 mM EDTA can completely inhibit MMP activity.

2. gel drying: The polyacrylamide gel drying device can be used to dry gel quickly at room temperature, which can be retained as a permanent record.

3. This reagent kit is only for scientific research experiments and cannot be used for other purposes such as consumption or clinical medicine.

During the experimental operation, please wear laboratory clothes, protective masks, and disposable laboratory gloves to avoid direct contact. Entry is strictly prohibited.

When using the gelatinase immunoassay kit, it is necessary to follow the steps in the instruction manual. Firstly, prepare the sample and conductSDS-PAGE electrophoresis separation, and then in a specific buffer system to restore the activity of MMPs in the sample, and hydrolysis of gelatin in the gel at their respective migration sites. Then, gel was dyed with Coomassie Brilliant Blue, and then decolorized. Finally, white bands were visible on the blue background. The strength of the bands was proportional to the activity of MMPs.The gelatin enzyme spectrum detection kit is suitable for scientific research in various fields such as biology, medicine, and pharmacology, such as tumor biology, cardiovascular disease, neurobiology, etc. For example, in tumor research, it is possible to detect the presence of tumor cellsThe activity of MMPs is used to evaluate the invasion and metastasis ability of tumors. In addition, the kit can also be used for drug screening and development, evaluating the efficacy and mechanism of drugs by detecting their impact on MMPs activity.It should be noted that the gelatinase immunoassay kit is only used for scientific research and cannot be used for diagnosis. At the same time, it is necessary to strictly follow the steps and safety regulations in the manual during the operation process to ensure the accuracy and reliability of the experimental results.