【Product Name】 ：Taipan Blue staining solution（0.4%）

：100ml

:4℃refrigeration

:12 months

:

Trypan blueTrypanblue, also known as trypan blue or trypanosome blue, has a molecular formula of C34H24N6Na4O14S4 and a molecular weight of 960.81, CASNumber72-57-1， Trypanblue is an anionic dye that cannot penetrate the normal and intact cell membrane of living cells, resulting in non staining of living cells and the so-called rejection phenomenon. However, the increased permeability of the cell membrane of dead cells allows the dye to enter the cell through the cell membrane, causing dead cells to color blue. It is the most commonly used method for detecting cell viability. It is generally believed that the loss of cell membrane integrity indicates that the cell has died, which is opposite to the effect of neutral red. Therefore, using trypan blue staining can easily and quickly distinguish between live and dead cells. Trypan blue is one of the most commonly used staining methods for identifying dead cells in tissue and cell culture.

Taipan Blue staining solution（0.4%）Commonly used to detect the integrity of cell membranes and whether cells are alive, live cells are not stained blue, and dead cells are stained light blue; Trypan blue can be engulfed by macrophages, making it a viable staining agent for macrophages; After staining with trypan blue, the cell survival rate can be accurately quantified by directly counting under a microscope or by taking photos and counting under a microscope,0.4% is the most commonly used concentration.

Taipan Blue staining solution（0.4%) is a cell reactive dye commonly used to detect the integrity of cell membranes and the survival rate of cells. It is one of the most commonly used methods for identifying dead cells in tissue and cell culture.

This product is only for scientific research experiments and is not intended for any other purposes!

[Precautions]:

1Taipan Blue staining solution（0.4%）Slightly irritating to the human body, please be careful with protection.

2Note that apoptotic bodies occasionally exhibit trypan blue rejection.

3For your safety and health, please wear lab coats and disposable gloves when operating.

The operation of Taipan Blue staining solution is simple and fast, usually only requiring3-5The dyeing process can be completed in minutes. When using trypan blue staining solution, a single-cell suspension is usually prepared first, and the cell suspension is mixed with trypan blue solution in an appropriate ratio (usually9:1）Then observe the staining of the cells and count them. The live cell rate and dead cell rate can be calculated by counting the number of live and dead cells, thus achieving a more accurate quantitative analysis of cell survival rate.