Cell: CNE-1 cell

Chinese name: Human nasopharyngeal carcinoma cells

Growth characteristics: adherent cells

Culture medium: 1640 Culture medium Serum: 10% FBS

Freezing conditions: 50% basic medium, 40% FBS, 10% DMSO

Routine cell culture and passage process (please strictly follow aseptic operation)

1. Suck out the culture medium from the original culture bottle, rinse the cells twice with PBS buffer, and add 2-3 ml of 0.25% trypsin for digestion of the cells (pay attention to the digestion time, usually controlled within 1-2 minutes)

2. Observe the digestion under the microscope. When the cell edge shrinks and the adhesion becomes loose (it is not recommended to digest until the cell floats), remove the pancreatic enzyme, add 6-8ml of culture medium, gently blow the cell layer, and try to blow it off and disperse as much as possible.

3. Transfer a portion of the cell suspension to a new culture dish/bottle, add appropriate culture medium, mix the cell suspension evenly, and culture it in the incubator.

4. Pay attention to changes in the pH value of the culture medium, change the medium regularly (2-3 times a week), and repeat one operation or freeze storage after the cell density reaches 80%.

(The online information is mainly for the purpose of online promotion and is for reference only. For cell culture, please consult the cell bank administrator. The information provided by the cell bank administrator shall prevail. Before operation, if there is anything unclear, please consult the cell bank administrator first to avoid unnecessary losses.)

Breast cancer is a common malignant tumor in women, and some patients may experience cancer recurrence and metastasis long after surgery. The recurrence of breast cancer after long-term dormancy is related to the action of specific substances in the bone marrow.

(Human HepG2 cell source: Tongpai Cell Bank human liver cancer cells)

Breast cancer patients who undergo surgical treatment may experience recurrence 10 or even 20 years later. Previously, it was believed that this was due to cancer stem cells moving into the bone marrow and entering a dormant state, which then revived and proliferated after a long period of time. But its sleep and recovery mechanisms have not been discovered yet.

(Source of Mouse Neuro-2a Cells: Mouse Brain Neuroma Cells from the Tongpai Cell Bank)

The study cultured mesenchymal stem cells from bone marrow together with breast cancer cells and found that when the "exosomes" with a diameter of about 100 nanometers (1 nanometer is one billionth of a meter) secreted by mesenchymal stem cells are absorbed by breast cancer cells, the proliferation of cancer cells will stop, and breast cancer cells will acquire stem cell like properties and enter a dormant state.

(Source of human IMR-32 cells: Tongpai Cell Bank human neuroblastoma cells)

Exosomes are extremely small vesicles that have the function of selectively removing some cell membrane proteins. Research has found that small ribonucleic acids (miRNAs) contained in exosomes are absorbed by breast cancer cells, causing changes in their gene expression and inducing them to enter a dormant state. Small ribonucleic acid is a type of single stranded ribonucleic acid molecule that does not encode proteins and mainly participates in controlling gene expression.

(Source of human CHP-100 cells: Tongpai Cell Bank human neuroblastoma cells)

After cancer cells become dormant, chemotherapy drugs find it difficult to exert their effects on them, and when these dormant cancer cells remaining in the bone marrow become active again due to certain circumstances, it can lead to breast cancer recurrence.

(Human WERI-Rb-1 cell source: human retinal neuroma cells from the Tongpai cell bank)

By studying the bone marrow of breast cancer patients, it was confirmed that breast cancer cells are adjacent to mesenchymal stem cells, and the latent cancer cells in the bone marrow contain significantly more small ribonucleic acids that induce dormancy compared to the primary lesion breast cancer cells.

(Source of Mouse YAC-1 Cells: NK Target Cells of Leukemia Cells from Tongpai Cell Bank)

This discovery can help monitor the recurrence and metastasis of breast cancer, and promote the development of treatment methods and drugs to prevent breast cancer recurrence.