22RV1 human prostate cancer cells

Cell properties:

Cell Introduction:

Cell name:22RV1 human prostate cancer cells

Cell nickname: 22RV1; 22Rv-1; 22rV1; CWR22-Rv1; CWR22R-V1; CWR22-R1; CWR22Rv1; CWR22R； Human prostate cancer cells

Product Code:

Species source: Human

Age and gender: Male

Organizational source: Prostate

Growth characteristics: Wall attached growth

Cell morphology: Epithelial like

Background introduction: Prostate cancer cells, which can produce retroviruses, require cell manipulation in a biosafety level 2 laboratory environment. 22RV1 cells express prostate-specific antigen PSA. Dihydroxytestosterone can slightly stimulate cell growth, and Western blot analysis shows that the dissolved products have an immune response to androgen receptor antibodies. EGF can stimulate cell growth, but TG β -1 cannot inhibit cell growth. Recently, it has been confirmed that 22RV1 can produce high titer human retrovirus XMRV. It can form tumors in nude mice.

Biosafety level: 2

Cell specifications: packaged in 1 × 106 cells/T25 culture bottles or 1mL cryovials

Mycoplasma testing: none

Gene expression status

Preservation institution: ATCC; CRL-2505 BCRC; 60545 DSMZ; ACC-438

Culture medium: 90% DMEM+10% FBS+PS

Cultivation conditions: Gas phase: 95% air+5% carbon dioxide; Temperature: 37 ℃

Freezing conditions: Serum free freezing solution, stored in liquid nitrogen

Doubling time:~40 hours

STR authentication location: Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,12 D16S539: 12 D5S818: 11,12,13 D7S820: 9,10,11 TH01: 6,9.3 TPOX: 8 vWA: 15,21

Cultivation precautions:

1. After receiving the cells, first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please contact us in a timely manner.

2. Carefully read the cell manual to understand the relevant information of the cell, such as cell morphology, culture medium used, serum ratio, required cytokines, etc., to ensure consistent cell culture conditions. If any problems occur due to inconsistent culture conditions, the responsibility shall be borne by the customer.

3. Wipe the surface of the cell vial with 75% alcohol and observe the cell state under a microscope. Due to transportation issues, it is normal for some cells to form fragments due to temperature changes and severe collisions. After observing the cell state, disinfect the bottle wall with 75% alcohol and place the T25 bottle in a 37 ℃ incubator for 2-4 hours

4. Adherent cells can be digested, suspended cells are directly mixed and collected, centrifuged at 900 rpm to 1000 rpm for 3 minutes, and the supernatant is discarded. Add 5 mL of PBS to resuspend the cells, centrifuge at 900 rpm to 1000 rpm for 3 minutes, resuspend the cells in fresh culture medium, and inoculate them into new culture bottles or dishes for cultivation in an incubator.

5. Please ask the customer to use the same culture medium for cell culture under the same conditions.

6. We suggest that customers take several photos of the cells in the first 3 days after receiving them, recording the cell status for communication and exchange with our technical department. Due to transportation reasons, some sensitive cells may experience instability. Please contact us promptly to inform us of the specific situation of the cells, so that our technical personnel can track and follow up until the problem is resolved.

7. This cell is for scientific research purposes only.

8. Note: The transport medium (infusion medium) cannot be used to culture cells anymore. Please use a newly prepared medium according to the instructions for cell culture conditions to culture cells. The recommended ratio for the first passage after receiving the cells is 1:2 passage.

9. Note: 1:2 passage refers to transferring one T25 bottle to two T25 bottles or two 6cm dishes. Not one T25 bottle to two 10cm dishes.

Processing after cell reception:

1) After receiving the cells, disinfect the bottle wall with 75% alcohol and place the T25 bottle in a 37 ℃ incubator for about 2-3 hours. If you find any damage to the culture bottle, overflow of liquid, or contamination of the cells, please take photos and contact us promptly.

2) Please confirm the cell status under a 4 or 5X microscope, and take 2-3 photos (10 ×, 20 ×) of the newly received cells, as well as one photo of the appearance of the culture bottle, for retention as a basis for the cell status upon receipt during after-sales service.

3) Adherent cells: Cells are placed in a 37 ℃ incubator for 2-3 hours, and their growth and adherence are observed under a microscope. Some adherent cells may detach and form clusters due to vibration during express delivery. If the growth density of cells observed under the microscope is below 60%, the culture medium in the culture bottle can be removed (if there are non adherent cells, they need to be centrifuged and resuspended into the original culture bottle), and 6-8mL of newly prepared * culture medium can be added and placed in the cell culture box for further cultivation. If the cell growth density reaches 70% -80% or more, the cells can be passaged. During the passage process, if cells shed due to transportation vibrations, they need to be recovered by centrifugation.

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