Glucose oxidase test kit 50 tubes/24 samples

Product attributes:

Product Name:Glucose oxidase test kit50 tubes/24 samples

Detection method: Visible spectrophotometry

Product Category: Oxidation and Antioxidant Series

Product Specifications50 tubes/24 samples

Detection method: Visible spectrophotometry

Product Code:BJ-01S9607

Product Introduction:

Required instruments and supplies:

Visible spectrophotometer1mL glass colorimetric dish (optical path 1cm), low-temperature centrifuge, pipette, mortar, ice, and distilled water

4、 Superoxide Dismutase（Determination of SOD:

Suggest selecting before the formal experimentMake predictions for 2 samples, understand the situation of this batch of samples, familiarize yourself with the experimental process, and avoid experiments

Waste of samples and reagents!

1. Sample preparation

4) Extract in a ratio of 500-1000:1 using an extraction solution (mL). ③ Liquid sample: direct detection; If turbid, centrifuge and take the supernatant for detection.
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Notes:

1. Reagent 2 is an enzyme and cannot be frozen. It should be placed on ice during use.

2. Only one care is needed.

3. If the absorbance value of the control tube is greater than 2, it is recommended to dilute reagent 2 with distilled water by 7 times before use (10 μ l of reagent 2 original solution+60 μ l of distilled water).

4. Why are some sample measuring tubes larger than the control tube for SOD, and what is the range of values for the control tube?

The scope of care is0.8-2. The low absorbance value of the control tube may be due to (1) reagent two or reagent four not being prepared and used immediately; (2) Not adding reagents in order; (3) The reaction time is not sufficient, and can be extended (reaction time of 30 minutes can be extended to 40 minutes). The high absorbance value of the control tube may be due to reagent 2 not being diluted by the corresponding multiple according to the operating instructions.

If the measuring tube is larger than the control tube, it may be due to the significant influence of impurities in the sample. In order to reduce the impact of impurities, the sample extraction supernatant is usually diluted with distilled water or extraction solutionTesting 10 times later can usually make the measurement normal. Multiply by the corresponding dilution factor in the calculation formula.