Pine wood nematode rapid detection instrument

　　Pine wood nematode rapid detection instrumentDesign based on detection principles. It can accurately analyze the collected pine tree samples and quickly identify the presence of pine wood nematodes in the samples through specific detection techniques. This process does not require complex sample preprocessing, greatly reducing detection time and improving detection efficiency. In practical applications, the detector is easy to operate. Forestry workers only need to follow the operating instructions, collect an appropriate amount of pine tree tissue samples, such as pine branches, wood slices, etc., place them in the designated position of the detector, start the detection program, and obtain the detection results in a short period of time.

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1Pine wood nematode rapid detection instrumentInstrument usage

The rapid detection system for pine wood nematodes mainly includes processes such as isolation, DNA extraction, and fluorescence quantitative PCR detection. It can quickly complete the detection of pine wood nematodes, automatically display the detection results and print the detection report, without the need for manual interpretation, with an accuracy rate of over 99%, greatly improving the efficiency of pine wood nematode detection work. The field rapid detection system is equipped with a portable and mobile operation, providing convenience for the detection of pine wood nematodes in the field. The automated molecular detection system for pine wood nematodes is widely used in forestry, forest defense stations, customs, universities, scientific research institutions and other units.

2、 Instrument features

1. The system has high detection accuracy.

2. The intelligent pine wood nematode DNA rapid detection system is equipped with an automated detection program for pine wood nematode DNA, which does not require complicated program settings and can run automatically with one click. It can not only detect nematode separation solution, but also directly detect pine wood chips without separating nematodes.

3. The system detection task is easy to operate, time-saving and efficient, and suitable for forestry and forest defense departments at all levels. It only takes 60 minutes to complete the entire process including sample processing, DNA extraction, computer-based testing, and result interpretation.

4. By using amplification fluorescence technology, laboratory testing and identification requirements can be completed through the system host.

5. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

6. Built in 10 inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

7. Marlow high-quality Peltier refrigeration chips, combined with German high PT1000 temperature sensors and temperature control mode with electric resistance heating compensation edge, have a high heating rate of 7 ℃ and a high cooling rate of 5 ℃, greatly reducing experimental time.

8. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

9. Simple and intuitive software guidance, making it easy to start detection experiments.

3、 Technical parameters

Sample capacity: 48x0.2ml, supports 8-tube connection

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: high-performance imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

Hot cover temperature: 30 ℃ -108 ℃ (default 105 ℃, hot cover temperature adjustable)

Heating rate: 3.5 ℃/s (MAX)

Cooling rate: 2.5 ℃/s (MAX)

Temperature control accuracy: ± 0.1 ° C

Temperature uniformity: ± 0.25 ° C

Temperature accuracy: ± 0.1 ℃

Number of temperature control areas: Multiple points (2 points)

Number of gradients: 0

Gradient temperature range: none

Number of gradient holes: none

Excitation light source: high-power LED (maintenance free, long lifespan)

Excitation light wavelength range: 400-700nm

Testing component: Imported high-sensitivity photoelectric sensor

Excitation and detection of propagation medium: High temperature resistant professional optical fiber

Number of detection channels: Standard configuration includes 4 channels

Applicable dyes and probes:

First channel: 470/520 FAM, SYBR Green

Second channel: 530/570 HEX, JOE, VIC

Third channel: 580/610 ROX, CY3.5, Texas Red

Fourth channel: 630/670 CY5

Software function: With multiple qPCR data analysis capabilities, it can simultaneously detect 4 detection channels

Amplification curve,

Quantitative standard curve

Melting curve

Support HRM detection

Data export: Experimental results can be directly exported in the form of graphics and tables; The detailed data report includes experimental conditions, data coordinates and tables, and data analysis parameters.

Data formats: XLS, PDF, DOCX

Noise:<45 dB

Screen size: 10 inches (HD)

Touch screen: capacitive type

External USB: Supports data import and export

Hot cover: automatic pressure regulation

4、 Configuration List