Zebrafish embryonic fibroblast pac2

Zebrafish embryonic fibroblast pac2

Product Name: Zebrafish Embryo Fibroblast Pac2

Product Category: Other Cell Lines

Growth characteristics: Wall attached growth

Cultivation System: L15+10% FBS CO2 free culture, 28 ° or 21 ° C culture

Cell morphology: fibroblast like

Freezing conditions: serum-free cell freezing solution

Specification: 1 × 10 ^ 6 cells/T25 culture bottle

Item number: YS-01X8564

Adding a temperature protectant during cell cryopreservation can greatly improve the freezing effect. The commonly used cryoprotectant is DMSO, which is an osmotic protectant that can quickly penetrate cells, increase the permeability of the cell membrane to water, lower the freezing point, delay the freezing process, and allow intracellular water to escape from the cell before freezing, forming ice crystals outside the cell and reducing intracellular ice crystals, thereby reducing the damage of ice crystals to cells.

Preparation and disinfection of PBS (can also be used for other BSS, such as Hanks, D-Hanks solution preparation):
1. Dissolve to volume: Pour the medicine (NaCl 8.0g, KCl 0.2g, Na2HPO4 • H2O 1.56g, KH2PO40.2g) into a container
In a beaker with double distilled water, stir with a glass rod until fully dissolved, then pour the solution into a volumetric flask to accurately volume 1000ml and shake
Mix well to prepare a newly prepared PBS solution.
2. Transfer to solution bottle for disinfection: Pour PBS into the solution bottle (large hanging needle bottle), cover it with a rubber cap, and insert a needle into it
Disinfect the pot with 8 pounds for 20 minutes. After high-pressure disinfection, use sterilized distilled water to replenish the evaporated water.
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Zebrafish embryonic fibroblast pac2

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Other biological culture medium products of the company include:

T1N0 meat broth T1N0 Broth is used for the growth test of Vibrio cholerae (SN standard)
T1N3 Broth for Growth Test of Vibrio cholerae (SN Standard)
MCPC Medium for Vibrio Isolation and Culture (FDA Standard)
MCPC Medium Supplement: Add each bottle of mCPC medium additive to 100ml of mCPC medium
Selective isolation and cultivation of Vibrio parahaemolyticus using Vice hemolytic vibrio AGAR agar
Vice hemolytic vibrio increasing bacterial fluid
Used for the enrichment culture of Vibrio parahaemolyticus
Czapek Dox Agar is used for identifying and preserving strains of Penicillium and Aspergillus
Toxic Producing Medium is used for toxin production culture of Penicillium and Aspergillus
Potato Dextrose Agar (PDA) is used for counting mold and yeast (GB standard)
Zebrafish embryonic fibroblast pac2
Hela (cervical cancer cell) ATCC strain inquiry
PC-3 (human prostate cancer cell) ATCC strain inquiry
SGC7901 (gastric adenocarcinoma cell) ATCC strain inquiry
Inquiry for ATCC strain 786-0 (renal clear cell adenocarcinoma)
6T-CEM (T-cell leukemia) ATCC strain inquiry
Jecket (lymphoma cell) ATCC strain inquiry
JAR (embryonal trophoblast carcinoma cell) ATCC strain inquiry
PC-12 undifferentiated (rat adrenal pheochromocytoma) ATCC strain inquiry
WI-38 (human diploid embryonic lung cell) ATCC strain inquiry
THP-1 (human monocyte) ATCC strain inquiry
Metal instrument cleaning: Metal utensils should not be soaked in acid. When cleaning, you can first use detergent to scrub, then rinse with tap water, wipe with 75% alcohol, then rinse with tap water, then rinse with distilled water, and finally dry or air dry. Put it in an aluminum box and package it in a pressure cooker with 15 pounds of high pressure (30 minutes) for disinfection, then dry it for later use.