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Experimental principle of Folin phenol reagent
Date: 2025-12-25Read: 0


Product Name Folin phenol reagent

Product Specifications 500ml

Storage conditions Room temperature sealing

level BR

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Product Introduction

Folin phenol(Folin phenol reagent can be used to determine protein content, and there have been many reports of improved analytical procedures using this reagent for protein content determination in serum, antigen antibody precipitates, and insulin. Although this reagent is recommended for its high sensitivity and easy operation, and although it has considerable advantages for certain applications, its characteristics and limitations need to be understood before comprehensive development. The effects of changes in pH, reaction time and reactant concentration, allowable levels of commonly used reagents for protein processing, and interfering substances have been studied.

Experimental PrincipleThe phosphomolybdic acid phosphotungstate salt in Folin reagent is reduced by tryptophan and tyrosine residues in the protein, resulting in a color reaction: producing blue (tungsten blue+molybdenum blue). The intensity of blue is directly proportional to the protein content.

Precautions

1. For your safety and health, please wear lab coats and disposable gloves when operating.

2. This product is only for scientific research experiments and is not intended for any other purposes.

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