To draw the correct conclusion, we must strictly follow the requirements for experimental operations, especially the relevant precautions. Next, let me explain to everyonePrecautions for operating ELISA kit.

1The unnecessary Flat noodles in the test shall be immediately put back into the packaging bag, sealed and reserved to prevent deterioration.

2Unnecessary other reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.

3Use disposable suction tips to prevent contamination and extract stop liquids and substratesA. When using liquid B, avoid using a sampler with some metal.

4Use clean plastic containers to prepare washing solution. Mix the various components and samples in the reagent kit thoroughly before use.

5Reagents should be stored according to the label instructions and allowed to recover to room temperature before use. The diluted standard should be discarded and not retained.

6When washing the enzyme-linked immunosorbent assay (ELISA) plate, it should be thoroughly dried. Do not directly put the absorbent paper into the ELISA reaction well to absorb water.

7SubstrateA should evaporate to prevent long-term opening of the lid. Substrate B is sensitive to light and prevents prolonged exposure to light. Prevent touching with hands. After the experiment is completed, the OD value should be read immediately.

8The order of adding reagents should be common to ensure that all reaction plate wells are incubated at the same time.

9Incubate according to the time, amount, and sequence indicated in the instruction manual. (The content is for reference only. If you have any questions or encounter problems, please go online and we will be happy to serve you.)