Prepare the reagents required for the experiment according to the instructions in the reagent kit manual.Distilled or deionized water used in ELISA, including those used for washing, should be fresh and of high quality. The self-made buffer solution should be measured correctly using a pH meter. The test reagents taken out of the refrigerator should be used after the temperature and room temperature have reached equilibrium. The parts of the reagent kit that are not needed for this experiment should be promptly returned to the refrigerator for storage.

ELISA kitPreparation: For each cytokine, carefully read the instructions and pay attention to the details of each batch. Before using cytokines, centrifuge the reagent bottle instantaneously to retrieve as many cytokines as possible. According to the details in each batch of instructions, dissolve the freeze-dried cytokines. The linear range of the standard curve for the general test antigen can be obtained by diluting the standard sample 8 times in a 2-fold series from 2000pg/ml to 15pg/ml. The sensitivity can be improved within a certain range by using standard ELISA operating procedures, amplification kits, third class reagents, or changing the enzyme substrate system. To optimize sensitivity, it is recommended to incubate standard samples and specimens. If peroxidase is used as a colorimetric system, it is strictly prohibited to add sodium azide to the washing solution and diluent. Sodium azide can inhibit the activity of peroxidase ELISA kit enzymes.

can be used asThe ELISA kit can detect a wide range of specimens, including body fluids (such as serum), secretions (saliva), and excreta (such as urine and feces), to determine certain antibodies or antigen components. Some specimens of ELISA kits can be directly measured (such as serum and urine), while others require pre-treatment (such as feces and certain secretions). There are certain requirements for the collection time, methods, and preservation of these specimens.

ELISA kitThe operation varies depending on the formation of the solid-phase carrier, and in domestic medical testing, plate type dots are generally used. This article will describe the key points to note for each operation step of plate ELISA, including tube and magnetic ball ELSIA. Foreign reagents are used in conjunction with special instruments.