Capan-1 pancreatic cancer cell (Capan-1 cell line)

Capan-1 pancreatic cancer cell (Capan-1 cell line)

For specific questions regarding the source, instructions, and information requirements of the required cells, please consult the customer service of the cell bank. You will receive advice, guidance, and replies, providing cells that you can trust and ensuring the smooth progress of your experiment;

MLE 12 cell line: adherent cell culture medium, serum: 90% RPMI-1640, 10% FBS

MLFC cell line: adherent cell culture medium, serum: 90% DMEM high glucose, 10% FBS

MG-63 cell line: adherent cell culture medium, serum: 90% McCoy's 5A, 10% FBS

MFC cell line: adherent cell culture medium, serum: 90% RPMI-1640, 10% FBS

[MLFC cell line: adherent cell culture medium, serum: 90% DMEM high glucose, 10% FBS]

Pancreatic cancer cells (Capan-1 cell line)

The professional team of Tongpai Cell Bank Molecular Platform provides dual luciferase reporter gene testing:

The dual luciferase reporter gene detection system (firefly luciferase gene, sea kidney luciferase gene) is used to quickly, sensitively, and conveniently determine whether transcription factors can interact with target promoter fragments.

Dual luciferase reporter gene cell culture, co transfected cells default to 293T cells, special cells are provided separately.

RPMI1640, a commonly used reagent for cell culture:

(For more cell culture questions, please consult the cell administrator)

1) Add the culture medium powder to 2/3 of the volume of the culture medium in double distilled water, rinse the packaging bag 2-3 times with double distilled water, stir until the powder is completely dissolved, and add the medicine according to the instructions.

HBE cell culture conditions: 90% DMEM, 10% FBS adherent cells

2) Add 0.5ml of prepared penicillin streptomycin su solution to the culture medium using a syringe, so that the final concentration of penicillin streptomycin su is 100 units/ml.

HBMEC cell culture conditions: 90% DMEM, 10% FBS adherent cells

3) Adjust the pH to around 7.2 using carbon dioxide or bicarbonate. Dilute to 1000ml and shake well.

Capan-1 pancreatic cancer cell (Capan-1 cell line)

Tongpai Biotech provides (LPS induced lung injury model):

Pulmonary neutropenia is a unified pathological manifestation in diseases such as respiratory distress syndrome (ARDS), cystic fibrosis (CF), and chronic obstructive pulmonary disease (COPD). It is speculated that the aggregation and activation of neutrophils in the lungs lead to symptoms of pulmonary dysfunction, including increased airway response, pulmonary fibrosis, and excessive mucus secretion.

Lipopolysaccharide (LPS) is the main component of the cell wall of Gram negative bacteria. When entering animal lung tissue, LPS exhibits various functions of lung damage, including white blood cell accumulation in lung tissue, pulmonary edema, severe lung inflammation, and high mortality rate.

The characteristics of LPS induced acute lung injury rat and mouse models are the accumulation of pulmonary neutrophils and the production of inflammatory mediators. Therefore, this model is very helpful for studying the targeted mechanisms related to neutrophil aggregation and mediator release.

Intranasal administration of LPS can induce an increase in neutrophils in the lungs of mice with LPS induced lung injury model. The results showed pathological changes, such as significant neutrophilic granulocytes, increased cell count, and increased production of inflammatory mediators (such as TNF - α) in bronchoalveolar lavage (BAL) fluid.

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Human HIC human small intestine cancer cells, human F56 human adenocarcinoma cells, human WERI-Rb-1 human retinal glioma cells, human KMB-17 cells human embryonic lung diploid cells

Human HL human embryonic lung diploid cell, human ME-180 human cervical epithelial cancer cell, human Bcap-37 human breast cancer cell, human NCI-H226 [H226] human lung squamous cell carcinoma cell

Mouse WEHI 164 cells, mouse fibrosarcoma cells, mouse J774A. 1 mononuclear macrophages, mouse C8-D1A cerebellar tissue cells, human A3 human T lymphocyte leukemia cells

L5178Y TK+/- mouse lymphoma cells, human CaES-17 human esophageal cancer cells, human KHM-5M human undifferentiated thyroid emulsion cancer cells, mouse M2-10B4 cells, mouse bone marrow fibroblasts

Mouse MA-782 cell mouse breast cancer cell, human FaDu human pharyngeal squamous cell carcinoma cell, rat H4- Ⅱ E cell rat hepatoma, human Daoy human medulloblastoma cell

Human HCC 94 [HCC 941122] human uterine squamous cells (highly differentiated), mouse U14 GFP cells, mouse cervical cells, human hUMSC human umbilical cord blood mesenchymal stem cells

Human K562/ADM chronic myeloid leukemia cell line resistant to drugs