ST cells (ST passage cell bank)

ST cells (ST passage cell bank)

Please consult the customer service of the cell bank for specific questions such as the source, description, and information requirements of the required cells; You will receive advice, guidance, and responses, providing cells that you can trust and ensuring the smooth progress of your experiment;

MLE 12 cell line: adherent cell culture medium, serum: 90% RPMI-1640, 10% FBS

MLFC cell line: adherent cell culture medium, serum: 90% DMEM high glucose, 10% FBS

MG-63 cell line: adherent cell culture medium, serum: 90% McCoy's 5A, 10% FBS

MFC cell line: adherent cell culture medium, serum: 90% RPMI-1640, 10% FBS

[MLFC cell line: adherent cell culture medium, serum: 90% DMEM high glucose, 10% FBS]

Human WERI-Rb-1 human retinal glioma cells

Human KMB-17 cells, human embryonic lung diploid cells

Human HL human embryonic lung diploid cells

Daoy human medulloblastoma cells

Human HCC 94 [HCC 941122] human uterine squamous cells (highly differentiated)

Mouse U14-GFP cells Mouse cervical cells

Human hUMSC human umbilical cord blood mesenchymal stem cells

Human K562/ADM chronic myeloid leukemia cell line resistant to drugs

ST cells (ST passage cell bank)

Tongpai Biotech provides (animal model of digestive system diseases):

high-fat diet/STZ induced diabetes model

Type 2 diabetes is a chronic metabolic disorder characterized by hyperglycemia, insulin resistance and insulin deficiency. It is highly valuable to develop stable experimental animal models in order to further elucidate the pathological characteristics of this complex disease, search for better treatment methods and new prevention strategies. The rat model fed with high-fat diet and injected with streptozotocin (HFD/STZ) is a very stable rodent model of type 2 diabetes.

Insulin resistance isOne of the important characteristics of type 2 diabetes. At the same time, large doses of streptozotocin (STZ) are widely used to induce type 1 diabetes in mice. However, low-dose STZ can cause slight impairment of insulin secretion, which is similar to the characteristics of the late stage of human type 2 diabetes. Based on the above characteristics, researchers have begun to develop a new diabetes model by combining diet (high fat or high fructose diet) and STZ treatment.

Firstly, feeding rats with the aforementioned special diet initially leads to hyperinsulinemia and insulin resistance. Subsequently, low-dose treatment was administered to the ratsSTZ injection leads to pancreatic beta cell damage and hyperglycemia. A rodent diabetes model that is very similar to human type 2 diabetes is obtained. Compared with other models, this model is cheaper and easier to operate. Therefore, it is very helpful to study and evaluate potential therapeutic drugs for type 2 diabetes.

The testing methods provided by Tongpai Biotechnology are as follows:

Weight monitoringOrganizational pathological evaluation, blood glucose level detection, food/water intake detection, fasting blood glucose concentration detection, serum insulin level detection, biomarker detection.

Common issues in cell culture:

1. How many stable passages can 661w cell lines be passaged?

2. How often do JEG3 cells divide?

3. Is C2C12 mouse cardiomyocytes easy to maintain?

4. Do SH-SY5Y cells grow slowly?

5. During the cryopreservation process of NK92MI cells, centrifugation is also involved, and it is not advisable to blow and scatter them too much. Is the entire cell mass frozen?

Detroit 562 human pharyngeal cancer pleural fluid metastatic cells

MS751 human cervical epidermal cancer cells

Human HB oral cancer cells

ME-180 human cervical epithelial cancer cells

Human Bcap-37 human breast cancer cells

Human NCI-H226 [H226] human lung squamous cell carcinoma cells

Mouse WEHI 164 cells Mouse fibrosarcoma cells

J774A. 1 Mouse mononuclear macrophages

Mouse C8-D1A cerebellar tissue cells

Human A3 human T lymphocyte leukemia cells

L5178Y TK+/- mouse lymphoma cells

CaES-17 human esophageal cancer cells

Human KHM-5M undifferentiated thyroid ductal carcinoma cells

Mouse M2-10B4 cells, mouse bone marrow fibroblasts

Mouse MA-782 cell Mouse breast cancer cell

FaDu human pharyngeal squamous cell carcinoma cells

Rat H4- Ⅱ E cell rat hepatocellular carcinoma

Hut-102 human T lymphoma cells

Human HIC human colon cancer cells

F56 human adenocarcinoma cells