Moc1/Moc2 Mouse Oral Squamous Cell Carcinoma/Female

Basic Information

Cell name：Moc1/moc2 mouse oral squamous cell carcinoma

Cell line：C57BL/6 Cxcr3-/-

Cell source：overseas

Cell identification：STR identification has been passed

cell morphology：Lymphatic polygonal cell like, adherent+suspended growth

Culture medium：DMEM (containing NaHCO3 1.5g/L) (BasMed-AW-013)+FBS 10%+P/S1% 500ML medium: IMDM: F10/F12=2:1313ml: 157ml medium+FBS 10% (50ml)+2.5mg/500ml insulin+20ug/500ml+2.5ug/500ml EGF+P/S 1% dual antibody, 1%.

culture conditions：Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%

Cellular background：Oral squamous cell carcinoma (OSCC) is a prominent subset of head and neck cancer, which is one of the six common cancers. The main risk factor for OSCC is exposure to carcinogens, distinguishing this subgroup of head and neck cancer from human papillomavirus induced head and neck cancer. Despite progress in detection, surgery, chemotherapy, and radiation therapy, the prognosis of OSCC has remained stable for decades. In addition, at the time of diagnosis, about two-thirds of OSCC patients suffered from local advanced diseases, which led to an increase in incidence rate and mortality.

Cell usage: For scientific research purposes only

Precautions

1. MOC1 cells have particularly high activity and rapid growth rate. It is not recommended to have too high a passaging density. Instead, it is recommended to dilute the cells and spread them thinly. When the cells reach about 80% of their length and are passaged, it is difficult to digest them. It is recommended to add 0.25% EDTA and mix thoroughly to ensure that all cells come into contact. Place the cells in a culture box for digestion for about 3-4 minutes before taking them out

2. Beat the side of the culture vessel to help cells shed. The beating process takes about 2 minutes to shed most of the cells. If the shedding rate is not very high, you can also put it back in the incubator and continue to digest for 1-2 minutes before taking it out again for beating and shedding. Wait for 80-90% of the cells to fall off before terminating digestion. Centrifuge and resuspend, then re lay the bottle and disperse evenly.

3. The adhesion of MOC2 cells is similar to that of conventional cells but not particularly strong. The doubling cycle is not as fast as MOC1. Treat with conventional digestion methods.

Shipping at room temperature

After receiving the T25 bottle, disinfect it and place it in the incubator for 2-3 hours. Observe the density and condition, take 2-3 photos, and provide feedback to the sales team. Once the density meets the standard, it can be passaged. The initial passage ratio is 1:2. It is recommended to freeze one whole bottle into a 1ml cryovial and continue passaging the other bottle. Repeat the freezing process for 2-3 bottles before amplification for experimentation to prevent seed breakage in case of unexpected situations.

Dry ice shipmentTwo conventional cell shipping cryovials were used, one was revived and the other was kept as a backup. If the first one failed to revive, the second one was resuscitated strictly according to the manufacturer's requirements. If none of the resuscitations were successful, please keep a photo of the resuscitation and notify us immediately.

adherent cells

1. Discard the culture supernatant and rinse the cells 1-2 times with PBS that does not contain calcium or magnesium ions.

2. Add 0.25% (w/v) -0.53 mM EDTA to culture bottles (1-2mL for T25 bottles and 2-3mL for T75 bottles), digest at 37 ° C for 1-2 minutes (digestion time can be appropriately extended for difficult to digest cells), and then observe the cell digestion under a microscope. If most of the cells become round and fall off, quickly take them back to the operating table, tap the culture bottle a few times, and add 3-4ml of culture medium containing 10% FBS to terminate digestion.

3. Gently mix and aspirate, centrifuge at 1000RPM for 3-5 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly. Divide the cell suspension into new T25 bottles/6cm culture dishes in a ratio of 1:2, add 6-8ml of the new culture medium prepared according to the instructions to maintain the growth vitality of the cells, and subsequently subculture according to the actual situation in a ratio of 1:2~1:5.

4. Cell cryopreservation: After receiving the cells, it is recommended to freeze a batch of cell seeds during the first 3 generations of cultivation for subsequent experiments.

5. The transport medium (infusion medium) cannot be used to culture cells anymore. Please use a newly prepared medium according to the instructions for cell culture conditions to culture cells.

Suspended cells

Cells grown in suspension can be maintained in their growth state by adding culture medium to the culture bottle. Generally, maintaining a cell density of 1 × 10 ⁵~1 × 10 ⁶ cells/mL (different cells have different density requirements) can maintain normal cell growth. If necessary, the cell suspension can be collected in a centrifuge tube at 1000rpm and centrifuged for 5 minutes. Discard the supernatant, add 1-2mL of culture medium, resuspend and mix well. Then, divide the cell suspension into new T25 bottles at a ratio of 1:2 and add 6-8ml of new culture medium prepared according to the instructions to maintain the growth vitality of the cells. Subsequent passages should be carried out at a ratio of 1:2-1:4 according to the actual situation.

biosafety

1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal.

2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells. Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.