Analysis and Detection Manual for Human Leukocyte Activated Adhesion Factor (ALCAM) ELISA Kit

Analysis and Detection Manual for Human Leukocyte Activated Adhesion Factor (ALCAM) ELISA KitMainly used for scientific research, not for clinical diagnosis. Shanghai Yuanmu Biotechnology specializes in selling imported/domestic ELISA kits, antibodies, culture media, serum, staining solutions, standards, chemical reagents, providing ELISA kit user manuals, ELISA kit prices, and more,ELISA KitBrands, welcome to order!

Chinese name: Human leukocyte activated adhesion factor (ALCAM) ELISA kit

Alias: Human leukocyte activated adhesion factor (ALCAM) ELISA Kit

Supplier: Shanghai Yuanmu Biotechnology

Specification: 48t/96t

Storage: Low temperature storage at 2-8 ℃

Validity period: 6 months

ELISA kit components: enzyme-linked immunosorbent assay (ELISA) plate, reagents, standards, etc.

Detection scope:
Human, sheep, mice, rats, pigs, rabbits, goats, cows, horses, pigs, other animal cytokines, plant cytokines, bone metabolism, cell apoptosis, hormone endocrine, active peptides, liver fibrosis, autoantibodies, thrombosis and hemostasis, tumors, autoantibodies research Elisa detection kit.

Analysis and Detection Manual for Human Leukocyte Activated Adhesion Factor (ALCAM) ELISA KitSample processing and requirements for this reagent kit:
1. Serum: Blood naturally coagulates at room temperature for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 revolutions per minute). Carefully collect the supernatant. If precipitation occurs during storage, centrifuge again.
2. Plasma: EDTA or sodium citrate should be selected as anticoagulants according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If sediment forms during storage, it should be centrifuged again.
3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If sediment forms during storage, centrifuge again. Refer to the implementation for pleural effusion, ascites, and cerebrospinal fluid.
4. Cell culture supernatant: When detecting secretory components, collect them using sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. When detecting intracellular components, dilute the cell suspension with PBS (pH 7.2-7.4) to a cell concentration of around 1 million/ml. By repeatedly freezing and thawing, cells are destroyed and intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.
5. Tissue specimen: After cutting the specimen, weigh it. Add a certain amount of PBS, pH 7.4. Quickly freeze and store in liquid nitrogen for future use. The specimen remains at a temperature of 2-8 ℃ even after melting. Add a certain amount of PBS (pH 7.4) and homogenize the specimen thoroughly by hand or using a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.
6. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided.
7. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).

Analysis and Detection Manual for Human Leukocyte Activated Adhesion Factor (ALCAM) ELISA KitAdvantages of this reagent kit:
1. Using fully imported raw materials and antibodies: efficient, sensitive, and specific, strictly introducing technical standards for mass production.
2. Advanced optimization solution: high repeatability and reliability.
3. Technical service: professional, timely, patient.
4. Spot supply: Delivery from Jiangsu, Zhejiang, and Shanghai will take the next day, while delivery from other regions will take 3-5 days.

Technical principle of human leukocyte activated adhesion factor (ALCAM) ELISA kit:
(1). Solid phase immobilization of antigens or antibodies and enzyme labeling of antigens or antibodies.
(2). The antigen or antibody bound to the surface of the solid-phase carrier still maintains its immunological activity.
(3). Enzyme labeled antigens or antibodies retain both their immunological activity and enzyme activity.
(4). The tested specimen reacts with the antigen or antibody on the surface of the solid-phase carrier. Adding enzyme labeled antigens or antibodies also binds to the solid-phase carrier through reaction.
(5). At this point, the amount of enzyme on the solid phase is proportional to the amount of the test substance in the specimen.
(6). After adding the substrate to the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the analyte in the specimen. Therefore, qualitative or quantitative analysis can be performed based on the depth of the color. The measurement method has high sensitivity (pg ng/ml level) and good repeatability. A highly sensitive experimental technique that combines the specific reactions of antigens and antibodies with the efficient catalytic activity of enzymes on substrates, based on immunological reactions.

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