Isopropyl - β - D-thiogalactoside, CAS:367-93-1

Chinese name: Isopropyl - β - D-thiogalactoside
CAS number: 367-93-1
English name: Isopropyl-beta-D-thiogalactopyranoside
EINEC:206-703-0
Molecular formula: C9H18O5S
Molecular weight: 238.3
Appearance: White crystalline powder
Solubility: soluble in water, methanol, ethanol, soluble in acetone, chloroform, insoluble in ethyl alcohol

Usage: IPTG is commonly used in cloning experiments that require induction of β - galactosidase activity. It is often used in combination with X-Gal or Bluo Gal for blue white screening of recombinant bacterial colonies that can induce the expression of lac operon in E. coli. IPTG binds to lacI repressor protein and changes its conformation to exert its effect, preventing inhibition of the β - galactosidase encoding gene lacZ.

Isopropyl - β - D-thiogalactoside, CAS:367-93-1Common knowledge of using reagents:
(1) Reagents should not come into contact with hands (some reagents have strong corrosiveness and other characteristics).
(2) Clean spoons, measuring cylinders, or droppers should be used to collect reagents, and it is strictly prohibited to use the same tool to continuously collect multiple reagents at the same time. After taking one reagent, the tool should be washed (the medicine spoon should be wiped dry) before using another reagent.
(3) After using the reagent, be sure to tightly seal the bottle cap and dropper. Do not put the wrong bottle cap or dropper in the wrong place. Do not wear it with loose ends. After use, please put the bottle back in its original place in a timely manner to avoid forgetting and inconvenience.
(4) The extracted reagents cannot be returned to the original reagent bottle (for fear of re contamination of the original reagents).

Isopropyl - β - D-thiogalactoside, CAS:367-93-1Processing prompt:
1. After purchasing, please make sure to confirm the precautions on the label.
2. Take measures and management systems to prevent inversion and breakage.
3. Confirm the precautions on the label again before use. Take necessary safety measures.
4. Operations that do not indicate their hazardous or harmful characteristics should also be carried out with caution.
5. Protective equipment must be worn and operated carefully.
6. Please refer to relevant regulations, literature, MSDS and other information to understand and master the characteristics of the reagent before carrying out safe operations.
7. Usually, when purchasing reagents, one should consider using them all at once. If there is a surplus due to insufficient estimation, more attention should be paid to its storage and management.
8. In case the reagent operator is not a professional technician. Operations must be carried out under the guidance and supervision of professionals.
9. For waste or old reagents after use, they should be disposed of in accordance with relevant laws and regulations.