EB virus nuclear antigen lgA antibody detection kit

EB virus nuclear antigen lgA antibody detection kit

Product description

This product is used for in vitro qualitative detection of EB virus nuclear antigen (EBNA1) IgA antibodies in human serum.

96 servings per box

Main components

1. Enzyme linked plate (coated with recombinant EB virus nuclear antigen expressed in E. coli system) 96 wells

2. Sample XI release solution (containing sodium casein) 1 bottle (12ml)

3. Enzyme conjugate (containing goat anti human multi antibody IgA HRP) 1 bottle (12ml)

4. Negative control (including normal human serum) 1 bottle (1.0ml)

5. Positive control (OD ≥ 1.500, containing human EBNA1/IgA antibody positive serum, 0.02M PBS, newborn bovine serum) 1 bottle (1.0ml)

6.20 times concentrated wash solution (PBST) 1 bottle (30ml)

7. Color reagent A (containing H2O2) 1 bottle (6ml)

8. Color reagent B (including TMB) 1 bottle (6ml)

9. Termination solution (2M H2SO4) 1 bottle (6ml)

10. One self sealing bag

11. Three patches of sealing film

Storage conditions and expiration date

Stored at 2-8 ℃, with a shelf life of 12 months.

After opening the bottle, store the reference substance at 2-8 ℃ and use it up within 2 weeks.

Production date and expiration date: see label.

【Limitations of testing methods】

1. Any positive result needs to be confirmed after contacting clinical information;

2. This reagent kit cannot be used as a quantitative reagent.

3. Used for the detection of human serum or plasma samples, do not use for the detection of saliva, urine, or other bodily fluids.

4. All immune experimental systems are inevitably non-specific, which may result in biological false positives.

【Precautions】

1. This product is only used for in vitro diagnosis, and the operation should be strictly carried out according to the instructions. The sealing film cannot be reused. Different batches of enzyme-linked immunosorbent assay (ELISA) plates, ELISA reagents, and positive and negative controls cannot be mixed, and cannot be mixed with reagents from other manufacturers.

2. Before use, please equilibrate the reagent kit to room temperature (approximately 30 minutes). Before the experiment, gently shake and mix the liquid reagent, and immediately seal it and store it at 2-8 ℃ after use. The unused microporous Flat noodles shall be sealed with desiccant in a self sealing bag and stored at 2~8 ℃. Do not use expired reagents.

3. When adding liquid, a sampler must be used and the accuracy of the sampler should be checked regularly. When adding different samples or reagent components, the pipette tip and sample slot should be replaced to prevent cross contamination.

4. When washing, each well should be filled with washing solution to prevent the free enzymes in the well from being washed away. When using a washing machine, a soaking time of 15-20 seconds should be set. After washing the plate, the next step must be carried out immediately and the enzyme-linked immunosorbent assay plate must not be left dry or agitated. Avoid long interruptions in the testing process to ensure uniform testing conditions for each hole.

5. The result judgment must be based on the reading of the enzyme-linked immunosorbent assay (ELISA) reader. When reading the results, the bottom of the enzyme-linked immunosorbent assay (ELISA) plate should be wiped dry and there should be no bubbles in the well. Do not touch the outer wall at the bottom of the hole, as fingerprints or scratches may affect the reading of the plate hole.

6. The components of the reagent kit contain reagents that are harmful to the human body to varying degrees. Be careful not to splash these components onto the eyes, skin, or clothing. Once this occurs, immediately clean the affected area with sufficient water. All samples (including negative and positive controls), waste liquids, and waste materials used should be treated as infectious agents.

7. When developing color, it is necessary to add developer A solution first and then developer B solution to avoid too low color development.

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