Hepatitis B virus surface antibody detection kit

Common name:Hepatitis B virus surface antibody detection kit(Colloidal gold method)

Card type: 25 people per box (single person packaging)

This product is used for in vitro qualitative detection of hepatitis B virus surface antibodies in human serum, plasma, or whole blood samples.

【 Principle of Inspection 】

This detection kit uses immune lateral chromatography. The surface antibody of hepatitis B virus in the test sample first binds to the recombinant hepatitis B virus surface antigen labeled with gold to form a complex. This complex swims forward and reacts with the pre coated recombinant hepatitis B virus surface antigen at the detection line (T), forming an antigen antibody gold labeled antigen complex. If the titer of hepatitis B virus surface antibody in the sample exceeds 10mIU/ml, a visible band appears to the naked eye; The gold labeled rabbit IgG polyclonal antibody binds to the coated goat anti rabbit IgG polyclonal antibody at the quality control line and shows a band; Negative samples only show bands in the quality control area.

Storage conditions and expiration date

1. Store this reagent at 2-30 ℃, away from light, heat, and dry.

2. The validity period of this reagent is 18 months.

3. The production date and expiration date can be found on the label.

1. Human serum/plasma or whole blood can be used as test samples. Anticoagulants (heparin, EDTA, sodium citrate) do not interfere with the test results.

Serum/plasma samples stored at 2-8 ℃ for refrigeration are recommended to be used within 72 hours; Whole blood samples are collected and used immediately; If long-term storage is required, serum/plasma samples should be directly frozen at -20 ℃. The recommended number of freeze-thaw cycles for frozen samples should not exceed 3 times, and the storage time should not exceed one year.

3. Hemolysis and lipid blood samples should be avoided, and contaminated samples should not be used.

:

1. Open the test tube, take out the test strip, and place it on a clean and horizontal table; If it is a detection card, open the aluminum foil bag and place the detection card on a clean and level tabletop.

2. Use a dropper to take 30 μ l of serum, plasma, or whole blood sample and drop it onto the test strip or detection card well, then add 2 drops (about 70 μ l) of sample diluent.

3. Set the timer and observe the results within 20 minutes after adding the sample. The results observed after 20 minutes are invalid.

After observing and recording the results, please discard the test strip or detection card to avoid confusion in the judgment of the results. If long-term preservation is required, please take photos.

Positive judgment value

1. Negative: The hepatitis B virus surface antibody in the sample is less than 10mIU/ml, and only one quality control line (C) appears.

2. Positive: The hepatitis B virus surface antibody in the sample is ≥ 10mIU/ml. Under the premise of the quality control line (C) appearing, the detection line (T) appears.

3. Invalid: If no quality control line (C) is observed, regardless of whether there is a detection line (T) displayed, it is an invalid result and should be retested.

Explanation of Test Results

1. The result is positive, indicating the presence of hepatitis B virus surface antibodies in the body, with an antibody titer of not less than 10mIU/ml.

2. The result is negative, indicating the absence of hepatitis B virus surface antibodies or antibody titers below 10mIU/ml.