Plant Fructopolysaccharide Synthases (FBEs) ELISA Kit

Plant Fructopolysaccharide Synthases (FBEs) ELISA Kit

Brand: Baili Lai

Item No.BLL105469E
Specification: 96t/48t
This product is suitable for scientific research experiments but not for clinical use
This kit is used for quantitative detection of serum, plasma, tissue homogenate, and related liquid samples in vitro.
Samples: serum, plasma, tissue fluid, tissue homogenate, etc
Shanghai Bailili Biotechnology Co., Ltd. specializes in ELISA kits, ELISA reagents, culture media, serum, antibodies, proteases, and peptides

1. Principle: Immunoassay is a method that utilizes the high selectivity and specificity recognition and binding principle between specific antibodies and antigens or haptens to analyze and determine the target antibody or antigen. Enzyme linked immunosorbent assay (ELISA) is a type of immunoassay that consists of three parts: immune recognition, signal output, and data processing.

Step 2: Immunorecognition involves coating a polystyrene 96 well plate with antibodies, and then using the antibodies to recognize the antigen to be tested (usually protein biomarkers of diseases, viruses, bacteria, etc.). The antigen is adsorbed onto the surface of the 96 well plate from a complex test solution. Then, the recognition signal is output directly or indirectly using antibodies labeled with horseradish peroxidase (HRP), fluorescence, or radioactivity. Finally, the concentration of the target antigen in the test sample is calculated using information such as signal strength and concentration gradient of the standard sample.

3. Classification: According to the different methods of immune recognition and signal output, ELISA can be divided into double antibody sandwich method, direct immune competition method, non direct immune competition method, and so on. The double antibody sandwich method is the most common in commercial applications.

Plant Fructopolysaccharide Synthases (FBEs) ELISA Kit

Notes:

1. The kit should be balanced at room temperature for 15-30 minutes before use when taken out from the cold storage environment. If the enzyme coated plate is not used up after opening, the Flat noodles should be stored in a sealed bag.

2. There may be crystal precipitation in concentrated washing solution. When diluting, it can be dissolved by heating in a water bath, and washing does not affect the results.

3. Sample dispensers should be used for each step of sample addition, and their accuracy should be regularly checked to avoid experimental errors. The sample addition time should be controlled within 5 minutes. If there are a large number of specimens, it is recommended to use a sampling gun for sample addition.

4. Please make a standard curve and repeat the hole at the same time as each measurement. If the content of the substance to be tested in the specimen is too high (the oD value of the sample is greater than the OD value of the standard well), please dilute it with the sample diluent by a certain multiple (n times) before measuring. When calculating, please multiply it by the total dilution multiple (xnx5) at the end

5. The sealing film is only for one-time use to avoid cross contamination.

6. Please store the substrate away from light.

7. Strictly follow the instructions for operation, and the judgment of test results must be based on the reading of the enzyme-linked immunosorbent assay reader. 8. All samples, detergents, and various waste materials should be treated as infectious agents.

9. Components from different batch numbers of this reagent must not be mixed.

Disclaimers
1. The reagent kit is for research purposes only and should not be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.
2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.
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