Plant Methyl Jasmonate (MeJA) ELISA Kit

NegotiableUpdate on 01/12

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Nature of the Manufacturer: Producers

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Overview

Plant methyl jasmonate (MeJA) ELISA kit $r $n Sample collection, processing, and storage methods $r $n1. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. $r $n2. Tissue homogenate: Add an appropriate amount of physiological saline to the tissue and crush it. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant $r $n3. Storage: If the sample is not detected in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed

Product Details

Plant Methyl Jasmonate (MeJA) ELISA KitBrand: Baili Lai

Application: Quantitative detection

Validity period: 6 months

Composition of reagent kit	48 hole configuration	96 hole configuration	save
Instruction Manual	1 copy	1	R.T.
Sealing film	2 pieces (48)	2 pieces (96)	R.T.
sealed bag	1 piece	1 piece	R.T.
Enzyme labeled coated plate	1*48	1*96	2-8℃ 保存
Standard: 27ng/ml	0.5ml * 1 bottle	0.5ml * 1 bottle	2-8℃ 保存
Standard diluent	1.5ml * 1 bottle	1.5ml * 1 bottle	2-8℃ 保存
Enzyme linked immunosorbent assay (ELISA) reagents	3ml * 1 bottle	6ml * 1 bottle	2-8℃ 保存
Sample diluent	3ml * 1 bottle	6ml * 1 bottle	2-8℃ 保存
Color reagent A liquid	3ml * 1 bottle	6ml * 1 bottle	2-8℃ 保存
Color developer B solution	3ml * 1 bottle	6ml * 1 bottle	2-8℃ 保存
Termination liquid	3ml * 1 bottle	6ml * 1 bottle	2-8℃ 保存
Concentrated washing solution	(20ml * 20x) * 1 bottle	(20ml * 30x) * 1 bottle	2-8℃ 保存

Operation process:

(1) The product is only used for adding 100 μ l of the test sample to each well of the scientific research test sample, with 3 parallel wells for each type of sample; Set up two negative control wells and add 100 μ l of untreated cell lysate to each well; Set up another blank control well and add 100 μ l of pure cell lysate.

(2) Place the enzyme-linked immunosorbent assay (ELISA) plate at 4 ℃ and coat overnight.

(3) Plate washing: Absorb the reaction solution in the well, rinse it once with washing solution (after filling the plate well with washing solution, shake it off), then fill the plate well with washing solution, soak for 1-2 minutes, and shake intermittently. Shake off the liquid inside the hole and pat dry on absorbent paper. Repeat washing 3-4 times.

植物茉莉酸甲酯（MeJA）ELISA试剂盒

(4) Add 50 μ l of PBS to each negative control well, and add 50 μ l of 1:500 diluted working solution to each sample well and blank well.

(5) Place the enzyme-linked immunosorbent assay (ELISA) plate in a wet box at 37 ℃ and incubate for 60 minutes.

(6) Wash the board, same as (4).

(7) Add 100 μ l of HRP labeled working solution diluted 1:5000 to each well.

(8) Place the enzyme-linked immunosorbent assay (ELISA) plate in a wet box at 37 ℃ and incubate for 60 minutes.

(9) Wash the board, same as (4).

(10) Add 100 μ l of TMB chromogenic solution to each well, gently mix for 10 seconds, and let it react in the dark at 37 ℃ for 15-20 minutes.

(11) Add 100 μ l of 2mol/L H2SO4 to each well to terminate the reaction.

(12) Measure the absorbance values W1 and W2 at 450nm and 630nm respectively, and the final measured OD value is the difference between the two (W1-W2) to reduce light interference caused by scratches or fingerprints on the container.

(13) Data processing: After obtaining the OD values of the specimen (S) and negative control (N), calculate the S/N value. S/N ≥ 2.1 is the positive judgment criterion.

Plant Methyl Jasmonate (MeJA) ELISA KitCommon usage knowledge:

(1) Reagents should not come into contact with hands (some reagents have strong corrosiveness and other characteristics).

(2) Clean spoons, measuring cylinders, or droppers should be used to collect reagents, and it is strictly prohibited to use the same tool to continuously collect multiple reagents at the same time. After taking one reagent, the tool should be washed (the medicine spoon should be wiped dry) before using another reagent.

(3) After using the reagent, be sure to tightly seal the bottle cap and dropper. Do not put the wrong bottle cap or dropper in the wrong place. Do not wear it with loose ends. After use, please put the bottle back in its original place in a timely manner to avoid forgetting and inconvenience.

(4) The extracted reagents cannot be returned to the original reagent bottle (for fear of re contamination of the original reagents).

植物茉莉酸甲酯（MeJA）ELISA试剂盒 Disclaimers

1. The reagent kit is for research purposes only and should not be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.

2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.