Plant NADP Malinase (NADP-ME) ELISA Kit

NegotiableUpdate on 03/08

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

The detection principle of the NADP malate enzyme (NADP-ME) ELISA kit for plants: The double antibody sandwich enzyme-linked immunosorbent assay is used. Add the specimen, standard sample, and HEP labeled detection antibody to the pre packaged captured antibody package in one go, incubate and wash. $r $n uses substrate nmE for color development, while TEB is converted to blue under the catalysis of chemical enzymes and to yellow under the action of acid. The depth of color is positively correlated with the reagents in the sample. Use an enzyme-linked immunosorbent assay (ELISA) reader to measure the absorbance at a wavelength of 450nm and calculate the sample concentration.

Product Details

Plant NADP Malinase (NADP-ME) ELISA Kit

Brand: Baili Lai

Item No.BLL105461E
Specification: 96t/48t
This product is suitable for scientific research experiments but not for clinical use
This kit is used for quantitative detection of serum, plasma, tissue homogenate, and related liquid samples in vitro.
Samples: serum, plasma, tissue fluid, tissue homogenate, etc
Shanghai Bailili Biotechnology Co., Ltd. specializes in ELISA kits, ELISA reagents, culture media, serum, antibodies, proteases, and peptides
植物NADP苹果酸酶（NADP-ME）ELISA试剂盒

1. Principle: Immunoassay is a method that utilizes the high selectivity and specificity recognition and binding principle between specific antibodies and antigens or haptens to analyze and determine the target antibody or antigen. Enzyme linked immunosorbent assay (ELISA) is a type of immunoassay that consists of three parts: immune recognition, signal output, and data processing.

Step 2: Immunorecognition involves coating a polystyrene 96 well plate with antibodies, and then using the antibodies to recognize the antigen to be tested (usually protein biomarkers of diseases, viruses, bacteria, etc.). The antigen is adsorbed onto the surface of the 96 well plate from a complex test solution. Then, the recognition signal is output directly or indirectly using antibodies labeled with horseradish peroxidase (HRP), fluorescence, or radioactivity. Finally, the concentration of the target antigen in the test sample is calculated using information such as signal strength and concentration gradient of the standard sample.

3. Classification: According to the different methods of immune recognition and signal output, ELISA can be divided into double antibody sandwich method, direct immune competition method, non direct immune competition method, and so on. The double antibody sandwich method is the most common in commercial applications. 植物NADP苹果酸酶（NADP-ME）ELISA试剂盒

Plant NADP Malinase (NADP-ME) ELISA Kit

Notes:

1. The kit should be balanced at room temperature for 15-30 minutes before use when taken out from the cold storage environment. If the enzyme coated plate is not used up after opening, the Flat noodles should be stored in a sealed bag.

2. There may be crystal precipitation in concentrated washing solution. When diluting, it can be dissolved by heating in a water bath, and washing does not affect the results.

3. Sample dispensers should be used for each step of sample addition, and their accuracy should be regularly checked to avoid experimental errors. The sample addition time should be controlled within 5 minutes. If there are a large number of specimens, it is recommended to use a sampling gun for sample addition.

4. Please make a standard curve and repeat the hole at the same time as each measurement. If the content of the substance to be tested in the specimen is too high (the oD value of the sample is greater than the OD value of the standard well), please dilute it with the sample diluent by a certain multiple (n times) before measuring. When calculating, please multiply it by the total dilution multiple (xnx5) at the end

5. The sealing film is only for one-time use to avoid cross contamination.

6. Please store the substrate away from light.

7. Strictly follow the instructions for operation, and the judgment of test results must be based on the reading of the enzyme-linked immunosorbent assay reader. 8. All samples, detergents, and various waste materials should be treated as infectious agents.

9. Components from different batch numbers of this reagent must not be mixed.

Disclaimers
1. The reagent kit is for research purposes only and should not be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.
2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.
Related products:

BLL105902EPlant Acetyl Hydroxy Acid Synthase (AHAS) ELISA Kit

BLL105903EPlant Galactinol ELISA Kit

BLL105904EPlant diglyceride diglyceride (DGDG) ELISA kit

BLL105905EPlant hop latent virus ELISA kit

BLL105906EPlant hop latent virus ELISA kit

BLL105907EPlant hop dwarf virus ELISA kit

BLL105908EPlant Exo - β -1,4-Glucanase ELISA Kit

BLL105909EPlant prenyltransferase ELISA kit

BLL105910EPlant heat shock protein (HSP90) ELISA kit

BLL105911EPlant Methyltransferase SDG8 (SDG8) ELISA Kit

BLL105912EPlant kaO2 hydroxylase ELISA kit

BLL105913EPlant Thiabendazole (TDZ) ELISA Kit

BLL105914EPlant derived chlorophyll ELISA kit

BLL105915EPlant Sweet Potato Chlorotic Spot Virus (SPCFV) ELISA Kit

BLL105916EPlant Sucralose Decomposing Enzyme (CIN) ELISA Kit