Standard calf serum

Standard calf serumProduct Introduction

Chinese name: Standard calf serum

Specification: 100ml/500ml/500ml * 20

Product Code: YXQ-392

Origin: Imported and Domestic

Product features: triple sterile filtration, radiation sterilization, new batch, long shelf life, good adhesion effect, high proliferation efficiency.

Yuanmu Biology's standard serumCow serum (sterilized by irradiation), horse serum, Bioind fetal bovine serum, and so on.

Storage method of serum:

(1) Long term stored serum must be stored in a low-temperature refrigerator at -20 ℃ -70 ℃. The storage time in a refrigerator at 4 ℃ should not exceed one month Due to the volume increase of about 10% when serum freezes, it is necessary to reserve a certain volume space before freezing it into a low-temperature refrigerator, otherwise contamination or glass bottle freezing may occur

(2) The serum provided by general manufacturers is sterile and does not require further filtration for sterilization If suspended particles are found in the serum, the serum can be added to the culture medium and filtered together. Do not filter the serum directly

(3) Serum thawing: The thawing of bottled serum requires a gradual thawing method: serum stored in a low-temperature freezer at -20 ° C to -70 ° C is dissolved in a refrigerator at 4 ° C for 1 day Then move to room temperature and wait for all to dissolve before packaging During the dissolution process, it is necessary to shake gently and evenly (be careful not to cause bubbles) to ensure uniform temperature and composition, and reduce the occurrence of precipitation Do not thaw serum directly from -20 ℃ to 37 ℃, as this can cause protein aggregation and precipitation due to significant temperature changes

(4) Heat inactivation refers to heating thawed serum at 56 ℃ for 30 minutes During the heating process, it is necessary to shake it regularly and evenly The purpose of this heat treatment is to inactivate complement components in serum Unless necessary, this heat treatment is generally not recommended as it can cause a significant increase in serum sediment