Standard newborn bovine serum

(1) Long term stored serum must be stored in a low-temperature freezer at -20 ℃ -70 ℃. Do not store in a refrigerator at 4 ℃ for more than one month. Due to the volume increase of about 10% when serum freezes, it is necessary to reserve a certain volume space before freezing it into a low-temperature refrigerator, otherwise contamination or glass bottle freezing may occur.

(2) The serum provided by general manufacturers is sterile and does not require further filtration for sterilization. If suspended particles are found in the serum, the serum can be added to the culture medium and filtered together. Do not filter the serum directly.

(3) Serum thawing: The thawing of bottled serum requires a gradual thawing method: serum stored in a low-temperature freezer at -20 ° C to -70 ° C is dissolved in a refrigerator at 4 ° C for 1 day. Then move to room temperature and wait for all to dissolve before packaging. During the dissolution process, it is necessary to shake gently and evenly (be careful not to cause bubbles) to ensure uniform temperature and composition, and reduce the occurrence of precipitation. Do not thaw serum directly from -20 ℃ to 37 ℃, as this can cause protein aggregation and precipitation due to significant temperature changes.

(4) Heat inactivation refers to heating thawed serum at 56 ℃ for 30 minutes. During the heating process, it is necessary to shake it regularly and evenly. The purpose of this heat treatment is to inactivate complement components in serum. Unless necessary, it is generally not recommended to perform this heat treatment as it can cause a significant increase in serum sediment.

Culture media can be divided into three categories based on their physical state: solid culture media, liquid culture media, and semi-solid culture media.

(1) Solid culture medium. It is to add coagulants such as agar, gelatin, silica gel, etc. to the culture medium. Solid culture medium is commonly used for microbial isolation, identification, counting, and strain preservation.

(2) Liquid culture medium. No coagulants are added to the liquid culture medium. The composition of this culture medium is uniform, and microorganisms can fully contact and utilize the nutrients in the medium, making it suitable for physiological research. Due to its high fermentation rate and convenient operation, it is also commonly used in the fermentation industry.

(3) Semi solid culture medium. It is a semi-solid state formed by adding a small amount of coagulant to the liquid culture medium. It can be used to observe the movement of bacteria, identify bacterial strains, and determine the potency of bacteriophages. Culture media can be divided into four categories based on the type of microorganisms: bacterial culture media, actinomycete culture media, yeast culture media, and mold culture media.

Fetal bovine serum 100ml/500ml

Standard fetal bovine serum 100ml/500ml

Class standard fetal bovine serum 100ml/500ml

Premium fetal bovine serum 100ml/500ml/500ml * 20

Crude fetal bovine serum 10000ml/pot

Insect cell fetal bovine serum 100ml

Newborn bovine serum 100ml/500ml

Standard newborn bovine serum 100ml/200ml/400ml/500ml

Superior newborn bovine serum 100ml/200ml/400ml/500ml

Premium newborn bovine serum 100ml/200ml/400ml/500ml

New bovine blood 100ml/200ml/500ml without fibers

Crude newborn bovine serum 10000ml/pot

Anticoagulant newborn bovine blood 100ml/200ml/500ml

Newborn cow plasma 100ml/200ml/500ml/500ml * 20 bottles